高通量测序技术发展的真是快啊,454最近发布的GS FLX Titanium XL+系统的测序长度已经可达700~1000bp了!!通量700M,运行时间23小时。资料请看这里。虽然454的通量有点低,成本有点高,但是它的读长实在是有点诱人。
下面这张表格综合比较了普通测序仪3730xl及Illumina, 454, Ion Torrent, SOLiD,PacBio RS等各种高通量测序仪的通量、读长及运行成本。
下面这张表格列出了各种高通量测序仪的仪器成本(单位是千美元)、产生的数据量,错误情况及其它一些信息。
相关文献:Glenn TC.Field guide to next-generation DNA sequencers. Mol Ecol Resour. 2011 Sep;11(5):759-69. doi: 10.1111/j.1755-0998.2011.03024.x. Epub 2011 May 19.
1F
楼主不简单啊,有神马打算写的我也可以帮忙~~~
B1
@ lishenghui 呵呵,谢谢。PLoB是一个生物信息资源分享的平台,非常欢迎您参与分享。其实PLoB就是一个公共的博客,每个注册的用户都可以直接写自己的一些经验或者自己看到的比较好的文章和资料。O(∩_∩)O~
2F
毕业论文准备补充一点儿关于测序方法的总结,查资料都查疯了。然后就看到了这个,太开心了
3F
有个问题,Illumina Hiseq怎么才600美刀一台?
B1
@ 怪羊基德 单位是千美元。。。
B2
@ lotus 额,原来如此.如果是600美刀,我都想买两台回家玩玩了
4F
怎么没有Illumina solexa测序仪的呢
B1
@ 易正鑫 solexa 是 Illumina 其他测序系统的基础
http://www.illumina.com.cn/technology/solexa_technology.asp
5F
楼主, 是哪里找到这么好的文献。
原文在那?
谢谢~
B1
@ allen 文献地址已经加上。
B2
@ ybzhao http://www.molecularecologist.com/next-gen-fieldguide/
2012 NGS Field Guide – Overview
We have updated the tables presented in Glenn (2011) for 2012 values. The 2011 tables are still available at 2011-field-guide-overview:
Table 1a-c. ”Grades” for common applications on various NGS instruments. Other information from the original table 1 is relatively static.
Table 2a. Run time, Millions of reads/run, Bases/read, and Yield/run for all common commercial NGS platforms.
Table 2b. Reagent costs/run, reagent costs/Mb, and minimum commercially available units for all common commercial NGS platforms.
Table 3a. List purchase price for for all common commercial NGS platforms, ancillary equipment, and service contracts.
Table 3b. Computational resources required for all common commercial NGS platforms.
Table 3c. Errors and error rates for common commercial NGS platforms.
Table 4. Advantages and Disadvantages for all common commercial NGS platforms.
Citation: Glenn, TC (2011) Field Guide to Next Generation DNA Sequencers. Molecular Ecology Resources. doi: 10.1111/j.1755-0998.2011.03024.x