在基因组学研究中,finish一个小的环形基因组,例如线粒体、叶绿体、质粒后,一般都会画一个全基因组的圈图,如下图: 最近我刚好在组装一个植物的细胞器,然后做了这个工作给大家推荐一个画圈图的工具OGD...
String Graph of a Genome
Regular readers are familiar with our explanation of de Bruijn graphs through the following steps - ...
String Graph Assembler
A recent Genome Research paper describing an innovative approach for assembling large genomes from N...
OrthoMCL使用详解
1.Orthomcl是用来干嘛的? 我们都知道,我们在注释完一些基因组后,会拿到大量的蛋白,而这些蛋白,孤零零的放在那,你不觉得别扭吗?除了常规的pfam、interpro做完了,再做点什么呢?聚个类...
MetaVelvet: a short read assember for metagenomics
Velvet是大家所熟知的一款基于二代数据进行基因组组装的软件了,PLoB中也有多篇相关的文章来介绍velvet的安装与使用了。MetaVelvet是否听说过呢。MetaVelvet是一款基于二代数据...
在de Bruijn Graphs中利用mate pair的信息
This is the last post in the de Bruijn graph series. Although we did not mention explicitly, each of...
Velvet的exp_cov参数介绍
Appropriate choice of the ‘exp_cov’ (expected coverage) parameter in Velvet is very important to get...
De Bruijn Graphs for Alternative Splicing and Repetitive Regions
Today we shall examine de Bruijn graphs for two structures that occur frequently in genomes or trans...
推荐一篇关于真核基因组注释方法与流程的文章
测一个未知基因组(de nove sequence),要进行测序、拼接及注释。关于测序仪和拼接软件已经讲的很多了,很少有关于基因组注释的文章。一篇最近在Nature Review Genetics上的...
K-mer应用实例分析
We started with 600 million 100mer reads from a genomic library and plotted its K-mer distribution. ...