This commentary is a continuation of previous one. We are adding some additional information in case...
Testing SOAPdenovo2 Pre-release Version II:pregraph_sparse
Our earlier commentary on SOAPdenovo2 provided a quick overview of an assembly. In the next few comm...
Testing SOAPdenovo2 Pre-release Version
Opening the zip file: The zip file ‘SOAPdenovo2.zip’ expanded into two programs - i) pregraph_sparse...
Howto make N50 better?
N50 statistic is widely used by bioinformaticians to judge the quality of their assemblies, but it i...
Running newbler: more de novo assembly parameters
There is a long list of options/flags/parameters for a newbler assembly, some of which have been tre...
Running newbler: de novo assembly
This post is about how to start up newbler for de novo assembly projects. I will describe setting up...
Newbler output VI: the ‘status’ files
The files that are the topic of this post are all tables, i.e. tab separated text files. The ‘status...
Newbler output V: the 454ContigScaffolds.txt and 454ScaffoldContigs.fna
In the post on what is new in newbler version 2.6, I introduced the -scaffold option. Briefly, with ...
Newbler output IV: on ultra-short and single-read contigs
Sometimes you might observe very short contigs, some even having high read depth. You might see thes...
Newbler output II: contigs and scaffolds sequence files, and the 454Scaffolds.txt file
The files most people are after when they do an assembly must be these: the actual contig and scaffo...