In the post on what is new in newbler version 2.6, I introduced the -scaffold option. Briefly, with ...

Sometimes you might observe very short contigs, some even having high read depth. You might see thes...

The files most people are after when they do an assembly must be these: the actual contig and scaffo...

With this post, I’ll start going through the output files newbler generates. Some of these will be d...

Since version 2.3, newbler has a -cdna option for de novo transcriptome assembly. In this post, I’ll...

The single file I’ll discuss today has in fact almost the entire assembly in it, besides the actual ...

I thought to start by explaining briefly how newbler works. I’ll do this by following the output new...

454生命科学公司所研发的新一代测序平台基于光纤微流体技术和包裹了待测DNA片段的乳化液滴技术（炸药中的表面活性剂来维持乳液的热稳定性）。基于焦磷酸测序法的超高通量基因组测序系统——Genome Se...

用454测序得到的序列用newbler拼接的效果最好，而用短序列拼接软件velvet拼接效果很差，所以不能将454的原始reads和Illumina产生的reads合到一起后用velvet进行拼接。在...