linux下用awk计算fasta序列的长度
fasta序列文件data.fa
- >Gorai.004G111100.1
- ATGGGTACTGCTCCAACCCAGTGCCCTTCTGGAATCACTGCAAATTTCCACGCCAAATTTGATAACAGAACTGAGTTTTC
- >Gorai.004G111100.2
- ATGTTTTTCATGCTCCGGTGGACAAGATACTCTGGGATGCCGGGGAACAGTTTTTCCTTTTCTTGGCAGACATATGCACATAAAATTCTT
- >Gorai.004G111100.3
- ATGGGTACTGCTCCAACCCAGTGCCCTTCTGGAATCACTGCAAATTTCCAC
- >Gorai.004G111100.4
- ATGGGAATGCATGAACTAGCAGCCAAAGTTGATGAGT
首先将fasta序列转换成一行显示,命令如下:
- awk '/^>/&&NR>1{print "";}{ printf "%s",/^>/ ? $0"%":$0 }' data.fa >data2.fa
结果:
- >Gorai.004G111100.1%ATGGGTACTGCTCCAACCCAGTGCCCTTCTGGAATCACTGCAAATTTCCACGCCAAATTTGATAACAGAACTGAGTTTTC
- >Gorai.004G111100.2%ATGTTTTTCATGCTCCGGTGGACAAGATACTCTGGGATGCCGGGGAACAGTTTTTCCTTTTCTTGGCAGACATATGCACATAAAATTCTT
- >Gorai.004G111100.3%ATGGGTACTGCTCCAACCCAGTGCCCTTCTGGAATCACTGCAAATTTCCAC
- >Gorai.004G111100.4%ATGGGAATGCATGAACTAGCAGCCAAAGTTGATGAGT
长度计算:
- awk -F"%" '{print $1"\t"length($2)}' data2.fa >data3.fa
结果:
- >Gorai.004G111100.1 80
- >Gorai.004G111100.2 90
- >Gorai.004G111100.3 51
- >Gorai.004G111100.4 37
1F
原创地址: http://tiramisutes.github.io/2015/11/08/fa-length.html
2F
不错 哈哈