PrimerX 是一款在线工具,用来设计突变PCR引物。PrimerX能够基于两种不同序列数据设计突变引物,一种可以输入突变的DNA序列,另一种是模板DNA编码的蛋白序列,另外还能够鉴定你设计的突变引物。
进入后可以点击以下三个选项进行操作:
Click on one of the tasks below to get started:
- I want to design a primer pair based on a mutation in my template DNA sequence.
- I want to design a primer pair based on a mutation in an encoded protein sequence.
- I want to characterize a primer that I have designed.
之后分别按照各自要求操作,提供或上传DNA或蛋白序列,突变位点,完成即可