FASTX-Toolkit

FASTX-Toolkit是一款用于处理Short-Reads FASTA/FASTQ文件的程序，里面包含了丰富的FASTA/FASTQ文件格式转换、统计等命令。

下面是其功能介绍：

FASTQ-to-FASTA converter （FASTQ转换成Fasta）
Convert FASTQ files to FASTA files.
FASTQ Information（FastQ质量统计图、核酸长度分布）
Chart Quality Statistics and Nucleotide Distribution
FASTQ/A Collapser
Collapsing identical sequences in a FASTQ/A file into a single sequence (while maintaining reads counts)
FASTQ/A Trimmer （去掉FASTA/FASTQ中barcode序列）
Shortening reads in a FASTQ or FASTQ files (removing barcodes or noise).
FASTQ/A Renamer（批量对FASTA/FASTQ序列重命名）
Renames the sequence identifiers in FASTQ/A file.
FASTQ/A Clipper（去掉FASTA/FASTQ中接头序列）
Removing sequencing adapters / linkers
FASTQ/A Reverse-Complement
Producing the Reverse-complement of each sequence in a FASTQ/FASTA file.
FASTQ/A Barcode splitter
Splitting a FASTQ/FASTA files containning multiple samples
FASTA Formatter
changes the width of sequences line in a FASTA file
FASTA Nucleotide Changer
Convets FASTA sequences from/to RNA/DNA
FASTQ Quality Filter
Filters sequences based on quality
FASTQ Quality Trimmer
Trims (cuts) sequences based on quality
FASTQ Masker
Masks nucleotides with 'N' (or other character) based on quality